Quick Overview for Blocking Buffer (2X) for Fluorescent Western Blotting (ABIN6953293)
应用范围
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Supplier Product No.
- mb-070-0050
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Supplier
- Rockland
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原理
- Blocking Buffer (2X) for Fluorescent Western Blotting
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纯化方法
- Blocking buffer is specifically formulated to achieve superior reproducible Western blotting images using this system.
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过滤
- Sterile filtered
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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应用备注
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Application Note: This product is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI)
Western Blot Dilution: User Defined
Other: Blocking solution can be diluted 1:1 in TBS or used undiluted.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2 X
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE.
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储存条件
- 4 °C
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储存方法
- Store blocking buffer at 4° C prior to opening. DO NOT FREEZE. Expiration date is six (6) months from date of receipt.
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有效期
- 6 months
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背景
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Synonyms: Multiplex Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer
Background: This blocking buffer is ideal for infrared Western blotting. Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophor
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